We work towards understanding the mechanisms of cellular reprogramming that governs the transformation of a somatic cell into a pluripotent one. We identify regulatory genes and gene products that govern the state of pluripotency. We use this knowledge to generate human isogenic pluripotent stem cells and to improve the efficiency of somatic cell nuclear transfer.
To Reach Us
Cellular Reprogramming Laboratory
Department of Animal Science B270 Anthony Hall Michigan State University East Lansing, MI 48824
Nuclear Transfer-Cloning can reprogram the nucleus of somatic cells to the extreme of bypassing the complex biological processes of gametogenesis and fertilization. Embryos thus reengineered can develop into live offspring ....(more)
Research
STEM CELLS
Embryonic Stem (ES) Cells are isolated from a preimplantation embryo at approximately 5 or 7 days after fertilization (human and Monkey respectively) By definition ES cells must be able to fulfill three characteristics ....(more)
Chemotherapy-induced late transgenerational effects in mice
To our knowledge, there is no report on long-term reproductive and developmental side effects in the offspring of mothers treated with a widely used chemotherapeutic drug such as doxorubicin (DXR), and neither is there information on transmission of any detrimental effects to several filial generations. Therefore, the purpose of the present paper was to examine the long-term effects of a single intraperitoneal injection of DXR on the reproductive and behavioral performance of adult female mice and their progeny. C57BL/6 female mice (generation zero; G0) were treated with either a single intraperitoneal injection of DXR (G0-DXR) or saline (G0-CON). Data were collected on multiple reproductive parameters and behavioral analysis for anxiety, despair and depression. In addition, the reproductive capacity and health of the subsequent six generations were evaluated. G0-DXR females developed despair-like behaviors; delivery complications; decreased primordial follicle pool; and early lost of reproductive capacity. Surprisingly, the DXR-induced effects in oocytes were transmitted transgenerationally; the most striking effects being observed in G4 and G6, constituting: increased rates of neonatal death; physical malformations; chromosomal abnormalities (particularly deletions on chromosome 10); and death of mothers due to delivery complications. None of these effects were seen in control females of the same generations. Long-term effects of DXR in female mice and their offspring can be attributed to genetic alterations or cell-killing events in oocytes or, presumably, to toxicosis in non-ovarian tissues. Results from the rodent model emphasize the need for retrospective and long-term prospective studies of survivors of cancer treatment and their offspring.
Epigenetic mechanisms regulate MHC and antigen processing molecules in human embryonic and induced pluripotent stem cells
Epigenetic mechanisms regulate MHC and antigen processing molecules in human embryonic and induced pluripotent stem cells. Suárez-Alvarez B, Rodriguez RM, Calvanese V, Blanco-Gelaz MA, Suhr ST, Ortega F, Otero J, Cibelli JB, Moore H, Fraga MF, López-Larrea C. PLoS One. 2010 Apr 16;5(4):e10192.
Cloned Cattle Can Be Healthy and Normal Lanza RP, Cibelli JB, Faber D, Sweeney RW, Henderson B, Nevala W, West MD, Wettstein PJ Science 2001 Nov 30;294(5548):1893-4
The health profile of cloned animals Cibelli JB, Campbell KH, Seidel GE, West MD, Lanza RP Nat Biotechnol 2002 Jan;20(1):13-4
The ethical validity of using nuclear transfer in human transplantation Lanza RP, Caplan AL, Silver LM, Cibelli JB, West MD, Green RM. JAMA 2000 Dec 27;284(24):3175-9
Prospects for the use of nuclear transfer in human transplantation Lanza RP, Cibelli JB, West MD Nature Biotechnology 1999 Dec;17(12):1171-4
Human Therapeutic Cloning Lanza RP, Cibelli JB, West MD Nature Medicine September 1999.
Cloning cattle in 1998: present and future opportunities Stice SL and Cibelli JB Proceeding for American Embryo Transfer Association. San Antonio Texas. October 1998.
New developments in nuclear transfer technology Stice SL and Cibelli JB Proceedings of the Annual Meeting Canadian Society of Animal Sciences. July 1998.
Somatic cell nuclear transplantation in cattle. The Cloning symposium: Reprogramming cell fate-transgenesis and cloning Robl JM, Cibelli JB, Stice SL The Institute of Reproduction and Development. Melbourne, Australia. April 1998.
Cloning: new breakthroughs leading to commercial opportunities Stice SL, Robl JM, Ponce de León FA, Jerry J., Golueke PG, Cibelli JB, Kaine JJ Theriogenology 49:129-138. 1998.
Letters & Popular Science
Letters
To the Honorable George W. Bush, President of the United States The Washington Post February 21, 2000
